精品人人搡人妻人人玩a片,最近2019中文字幕电影免费看,久久精品国产亚洲7777,年轻又漂亮的后妈2

咨詢熱線

15000266580

當前位置:首頁 >產(chǎn)品中心>細胞庫>倉鼠肺細胞>CRL-1657R 1610 倉鼠肺細胞

R 1610 倉鼠肺細胞

簡要描述:CRL-1657 R 1610 倉鼠肺細胞,ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞|貼壁細胞|懸浮細胞|,細胞庫管理規(guī)范,提供的細胞株背景清楚,提供參考文獻和*培養(yǎng)條件

  • 產(chǎn)品型號:CRL-1657
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2024-11-14
  • 訪  問  量:2046

產(chǎn)品分類

Product Category

詳細介紹

CRL-1657 R 1610 倉鼠肺細胞,ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞|貼壁細胞|懸浮細胞|,細胞庫管理規(guī)范,提供的細胞株背景清楚,提供參考文獻優(yōu)培養(yǎng)條件


CRL-1657 R 1610 倉鼠肺細胞 的詳細介紹

CRL-1657

ATCC® Number:



Shipped:  frozen CRL-1657™

Designations:  R 1610

Depositors:   M Rosenberg

Biosafety Level: 1

Medium & Serum:  See Propagation

Growth Properties: adherent

Organism: Cricetulus griseus (hamster, Chinese)

Morphology: fibroblast



Source: Organ: lung

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Applications: transfection host

Gender:  male

Comments: This line is a clonal derivative of V79 cells (see also V79-4, ATCC CCL-93) that had been treated with ethylmethanesulfonate.

The cells are deficient in galactokinase and are resistant to 2-deoxygalactose and 8-azaguanine.

This is a Chinese hamster somatic cell line.

Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing:  Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37°C.



Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:4 is recommended

Medium Renewal: 2 to 3 times per week

Preservation:  Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liqid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

parental cell line:ATCC CCL-93

References: 10111: Schumperli D, et al. Efficient expression of Escherichia coli galactokinase gene in mammalian cells. Proc. Natl. Acad. Sci. USA 79: 257-261, 1982. PubMed: 6281760

25665: Thirion JP, et al. Galactokinase mutants of Chinese hamster somatic cells resistant to 2-deoxygalactose. Genetics 83: 137-147, 1976. PubMed: 1269916

Related Links

NCBI Entrez Search

Make a Deposit

Frequently Asked Questions

Material Transfer Agreement

Technical Support

Related Cell Culture Products






















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細地址:

  • 補充說明:

  • 驗證碼:

    請輸入計算結(jié)果(填寫阿拉伯數(shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術支持:化工儀器網(wǎng)    管理登陸
啦啦啦资源视频在线观看8| 亚洲日本va中文字幕无码| 特大黑人与亚洲娇小| 老师办公室狂肉校花h| 精品白嫩BBWBBWBBW| 熟妇人妻久久中文字幕| 成人做受视频试看60秒| 猛烈h继攵禁忌h| 粗大的内捧猛烈进出的视频| 精品人妻伦一二三区久久| 亚洲日韩精品一区二区三区| 精品国产制服丝袜高跟| 被侍卫g到c吹的皇后| 国内老熟妇对白XXXXHD| 高清欧美性猛交xxxx黑人猛交| 女人爽到高潮潮喷叫床69| 女局长白白嫩嫩大屁股| 苍井空浴缸大战猛男120分钟| 韩国三级中文字幕HD久久精品| 中国熟妇xxxx| 高潮时粗俗不堪入耳的话 | 国产精品美女WWW爽爽爽视频| 成人区精品人妻一区二区不卡| 国产96在线 | 亚洲| 国产人妻精品一区二区三区不卡| 扒开双腿疯狂进出爽爽爽动态照片| 母亲5免费完整高清电视| 解开警花的裙子猛烈进入| 色欲AV伊人久久大香线蕉影院| 免费a片在线观看| 久久久久久精品成人免费图片| 成人午夜视频| 女朋友闺蜜奶好大下面好紧| 少妇人妻人伦a片| 东北体育生巨大粗爽gay| 中文精品无码中文字幕无码专区| 交换年轻夫妇HD中文字幕3D| 黑色包臀裙秘书啪啪久久网站| 美女露?0的奶头无挡挡| 亚洲av无码a片在线观看蜜桃| 国产成人精品一区二区A片带套|